One Hourglass Kit - Two Pathways


Fill · Centrifuge · Collect

Fill

Centrifuge

Collect

The hourglass is the only physical solution to the platelet separation issue.

Fill

The lateral port is used to carefully insert the blood sample (25 ml). It is also where, after centrifugation, you collect the quantity of desired plasma to produce the final formula.

The central port is the device's most ingenious feature allowing to concentrate the buffy-coat in 0,1 ml to 0,2 ml volume. By twisting the lower chamber counterclockwise, the red blood cells are hermetically isolated from the rest of the plasma.
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Fill

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The lateral port is used to carefully insert the blood sample (25 ml). It is also where, after centrifugation, you collect the quantity of desired plasma to produce the final formula.

The central port is the device's most ingenious feature allowing to concentrate the buffy-coat in 0,1 ml to 0,2 ml volume. By twisting the lower chamber counterclockwise, the red blood cells are hermetically isolated from the rest of the plasma.
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Spin

Platelets need to be handled with utmost care. The centrifuge step is critical in this respect. For optimal performance, we recommend the high-end TD5 centrifuge machine. It is so efficient that only 3 minutes are needed.

The machine is suitable for gentle, gradual centrifugation. Samples are placed horizontally during spinning, thus benefitting from the full centrifuge force – an option not available with a 45° rotor.

The device is controlled by a microprocessor with digital display and touchscreen technology. The cover has an automatic lock and protection against overheating and imbalance. Its performance is ultra-fast and noiseless. The high-grade stainless steel casing is safe and reliable. CE Marking, ISO 9001 & ISO 13485 approved.

Spin

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Platelets need to be handled with utmost care. The centrifuge step is critical in this respect. For optimal performance, we recommend the high-end TD5 centrifuge machine. It is so efficient that only 3 minutes are needed.

The machine is suitable for gentle, gradual centrifugation. Samples are placed horizontally during spinning, thus benefitting from the full centrifuge force – an option not available with a 45° rotor.

The device is controlled by a microprocessor with digital display and touchscreen technology. The cover has an automatic lock and protection against overheating and imbalance. Its performance is ultra-fast and noiseless. The high-grade stainless steel casing is safe and reliable. CE Marking, ISO 9001 & ISO 13485 approved.

Two Pathways

The WorldPRP tube is designed by a doctor for doctors. That's why it has unparalleled versatility to handle every clinical situation: those - the majority - requiring a lot of volume, and those that demand a certain concentration, i.e. platelets in a smaller volume.

Pathway 1: Volume

A.  Twist the bottom of Tube to push the BC gently above the neck line of the tube to mix it with the plasma in the upper chamber. Stop a little before the RBC reaches the neck line of the tube. Stir gently in order to resuspend the Buffy Coat.
B.  Close the top of the tube tight to seal the top and bottom chamber. 
C.  From lateral port, Aspirate the PRP and PPP with a or 10ml syringe without neddle. 

Obtained PRP: Volume 12 ml; Yield >80%; Concentration 2x.
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Pathway 1: Volume

A.  Twist the bottom of Tube to push the BC gently above the neck line of the tube to mix it with the plasma in the upper chamber. Stop a little before the RBC reaches the neck line of the tube. Stir gently in order to resuspend the Buffy Coat.
B.  Close the top of the tube tight to seal the top and bottom chamber. 
C.  From lateral port, Aspirate the PRP and PPP with a or 10ml syringe without neddle. 

Obtained PRP: Volume 12 ml; Yield >80%; Concentration 2x.
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Pathway 2: Concentration

A.  Twist the bottom of Tube to push the super rich plasma gently to the top line of the neck of the tube. 
B.  Close the top of the Tube tightly to seal the top and bottom chamber. 
C.  Insert a 1ml syringe in the middle port. Hold it firmly, keep twisting the bottom chamber. Retrieve the 1ml of super rich plasma.
D.  Mix with 4 ml of plasma extracted from the upper chamber (connector provided). 

Obtained PRP: Volume 5 ml; Yield >65%; Concentration 4x.

Pathway 2: Concentration

A.  Twist the bottom of Tube to push the super rich plasma gently to the top line of the neck of the tube. 
B.  Close the top of the Tube tightly to seal the top and bottom chamber. 
C.  Insert a 1ml syringe in the middle port. Hold it firmly, keep twisting the bottom chamber. Retrieve the 1ml of super rich plasma.
D.  Mix with 4 ml of plasma extracted from the upper chamber (connector provided). 

Obtained PRP: Volume 5 ml; Yield >65%; Concentration 4x.
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